Search results for "Plasma Proteins"

showing 7 items of 7 documents

Characterization of the porcine seminal plasma proteome comparing ejaculate portions.

2016

Full identification of boar seminal plasma (SP) proteins remains challenging. This study aims to provide an extensive proteomic analysis of boar SP and to generate an accessible database of boar SP-proteome. A SP-pool (33 entire ejaculates/11 boars; 3 ejaculates/boar) was analyzed to characterize the boar SP-proteome. Twenty ejaculates (5 boars, 4 ejaculates/boar) collected in portions (P1: first 10 mL of sperm rich ejaculate fraction (SRF), P2: rest of SRF and P3: post-SRF) were analyzed to evaluate differentially expressed SP-proteins among portions. SP-samples were analyzed using a combination of SEC, 1-D SDS PAGE and NanoLC-ESI-MS/MS followed by functional bioinformatics. The identified…

0301 basic medicineMaleProteomicsendocrine systemBOARProteomeSwineQuantitative proteomicsBiophysicsComputational biologyBioinformatik och systembiologiBiologyBioinformaticsBiochemistry03 medical and health sciencesTandem Mass SpectrometryAnimalsPorcine; Ejaculate; Seminal plasma; ProteomeEjaculationSperm qualityDatabases ProteinLabel freeBioinformatics and Systems Biologyurogenital systemProteomic ProfilingReproductionSeminal Plasma ProteinsComputational BiologySpermSemen Analysis030104 developmental biologyFertilityGene Expression RegulationBiological significanceProteomeElectrophoresis Polyacrylamide GelChromatography LiquidJournal of proteomics
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Efficacy and safety of bempedoic acid for the treatment of hypercholesterolemia: A systematic review and meta-analysis

2020

Background Bempedoic acid is a first-in-class lipid-lowering drug recommended by guidelines for the treatment of hypercholesterolemia. Our objective was to estimate its average effect on plasma lipids in humans and its safety profile. Methods and findings We carried out a systematic review and meta-analysis of phase II and III randomized controlled trials on bempedoic acid (PROSPERO: CRD42019129687). PubMed (Medline), Scopus, Google Scholar, and Web of Science databases were searched, with no language restriction, from inception to 5 August 2019. We included 10 RCTs (n = 3,788) comprising 26 arms (active arm [n = 2,460]; control arm [n = 1,328]). Effect sizes for changes in lipids and high-…

Apolipoprotein BPublication Ethics030204 cardiovascular system & hematologyCardiovascularGastroenterologyLipoprotein particleMedical and Health SciencesBiochemistrychemistry.chemical_compoundDatabase and Informatics Methods0302 clinical medicineMathematical and Statistical TechniquesAnticholesteremic Agents Apolipoproteins B Cholesterol Cholesterol LDL Clinical Trials Phase II as Topic Clinical Trials Phase III as Topic Dicarboxylic Acids Fatty Acids Humans Hypercholesterolemia Peptide Fragments Randomized Controlled Trials as TopicLipid and Blood Pressure Meta-Analysis Collaboration (LBPMC) Group and the International Lipid Expert PanelMedicine and Health SciencesDicarboxylic Acids030212 general & internal medicineDatabase SearchingResearch IntegrityRandomized Controlled Trials as Topicmedicine.diagnostic_testbiologyAnticholesteremic AgentsStatisticsFatty AcidsRDrugsGeneral MedicineMetaanalysisSerious Mental IllnessLipidsPhase III as TopicMental HealthCholesterolPhysical SciencesMedicineResearch Articlemedicine.medical_specialtyRMScience PolicyLipoproteinsHypercholesterolemiaBempedoic acid hypercholesterolemia lipid profile hsCRPResearch and Analysis MethodsLDL03 medical and health sciencesClinical Trials Phase II as TopicInternal medicineGeneral & Internal MedicinemedicineHumansClinical TrialsStatistical MethodsApolipoproteins BPharmacologyPlasma Proteinsbusiness.industryCholesterolPhase II as TopicStatinsBiology and Life SciencesProteinsOdds ratioCholesterol LDLConfidence intervalPeptide FragmentschemistryClinical Trials Phase III as Topicbiology.proteinUric acidCreatine kinaseLipid profilebusinessDigestive DiseasesMathematicsPLoS Medicine
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Direct quantification of cell-free, circulating DNA from unpurified plasma.

2014

Cell-free DNA (cfDNA) in body tissues or fluids is extensively investigated in clinical medicine and other research fields. In this article we provide a direct quantitative real-time PCR (qPCR) as a sensitive tool for the measurement of cfDNA from plasma without previous DNA extraction, which is known to be accompanied by a reduction of DNA yield. The primer sets were designed to amplify a 90 and 222 bp multi-locus L1PA2 sequence. In the first module, cfDNA concentrations in unpurified plasma were compared to cfDNA concentrations in the eluate and the flow-through of the QIAamp DNA Blood Mini Kit and in the eluate of a phenol-chloroform isoamyl (PCI) based DNA extraction, to elucidate the D…

Gene Identification and Analysislcsh:MedicineCoronary DiseaseReal-Time Polymerase Chain ReactionBiochemistrylaw.inventionMolecular Geneticschemistry.chemical_compoundDiagnostic MedicinelawNucleic AcidsMolecular Cell BiologyBlood plasmaGeneticsHumanslcsh:ScienceExerciseBiologyPolymerase chain reactionDNA PrimersPlasma ProteinsMultidisciplinaryBase SequenceCell-Free SystemChemistrylcsh:RProteinsDNAMolecular biologyDNA extractionCoronary heart diseaseReal-time polymerase chain reactionCase-Control StudiesRNAMedicineCirculating DNAlcsh:QGene expressionGene FunctionPrimer (molecular biology)DNA modificationDNAResearch ArticlePLoS ONE
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Studying Closed Hydrodynamic Models of "In Vivo" DNA Perfusion in Pig Liver for Gene Therapy Translation to Humans.

2016

17 páginas, 6 figuras. En la versión online contiene 3 figuras y 1 tabla en información suplemetaria

Male0301 basic medicineSwineCardiovascular ProceduresGenetic enhancementProtein ExpressionCellGene ExpressionMetal Nanoparticleslcsh:MedicineVascular SurgeryBiochemistryTranslational Research BiomedicalMice0302 clinical medicinePig ModelsGene expressionMedicine and Health SciencesTransgeneslcsh:ScienceMammalsMultidisciplinaryPhysicsGene Transfer TechniquesClassical MechanicsAgricultureAnimal ModelsPerfusionmedicine.anatomical_structureLivermedicine.veinOrgan SpecificityNaked DNA030220 oncology & carcinogenesisVertebratesPhysical SciencesFemalePerfusionPlasmidsResearch ArticleLivestockSurgical and Invasive Medical ProceduresFluid MechanicsBiologyGene deliveryResearch and Analysis MethodsContinuum MechanicsInferior vena cavaCatheterizationGene Delivery03 medical and health sciencesModel OrganismsIn vivoGene Expression and Vector TechniquesmedicineAnimalsHumansMolecular Biology TechniquesMolecular BiologyMolecular Biology Assays and Analysis TechniquesPlasma Proteinslcsh:ROrganismsBiology and Life SciencesProteinsFluid DynamicsDNAGenetic TherapyMolecular biology030104 developmental biologyalpha 1-AntitrypsinAmniotesHydrodynamicslcsh:QGoldPLoS ONE
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Distant Homology Modeling of LCAT and Its Validation through In Silico Targeting and In Vitro and In Vivo Assays

2013

LCAT (lecithin:cholesterol acyltransferase) catalyzes the transacylation of a fatty acid of lecithin to cholesterol, generating a cholesteryl ester and lysolecithin. The knowledge of LCAT atomic structure and the identification of the amino acids relevant in controlling its structure and function are expected to be very helpful to understand the enzyme catalytic mechanism, as involved in HDL cholesterol metabolism. However - after an early report in the late '90 s - no recent advance has been made about LCAT three-dimensional structure. In this paper, we propose an LCAT atomistic model, built following the most up-to-date molecular modeling approaches, and exploiting newly solved crystallog…

MaleModels MolecularProtein StructureDrug Research and DevelopmentProtein Conformationlcsh:MedicineBiologyBiochemistryCatalysisSubstrate SpecificityPhosphatidylcholine-Sterol O-AcyltransferaseMicechemistry.chemical_compoundEnzyme activatorTransacylationProtein structureDrug DiscoveryHydrolaseCatalytic triadBiochemical SimulationsMedicine and Health SciencesAnimalsHumansHomology modelingBiomacromolecule-Ligand Interactionslcsh:SciencePharmacologyBinding SitesPlasma ProteinsMultidisciplinarylcsh:RBiology and Life SciencesProteinsEnzyme structureEnzyme ActivationMolecular Docking SimulationchemistryBiochemistryMutationEnzyme StructureEnzymologyBiocatalysisCholesteryl esterlcsh:QResearch ArticleBiotechnologyPLoS ONE
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Filling the void about sperm function knowledge and how the -omics approach can close the circle.

2013

MaleSeminal Plasma ProteinsObstetrics and GynecologyFunction (mathematics)Computational biologyOligospermiaBiologyThe VoidBioinformaticsOmicsSpermOxidative StressReproductive MedicineAsthenozoospermiaHumansInfertility MaleFertility and sterility
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Mass spectrometric identification of the amino donor and acceptor sites in a transglutaminase protein substrate secreted from rat seminal vesicles.

1991

Four different transglutaminase-modified forms of a protein secreted by the rat seminal vesicles (SV-IV) were synthesized in vitro and characterized. FAB maps of both the native protein and its derivatives, produced by the purified guinea pig liver enzyme in the presence or absence of the polyamine spermidine, were obtained by mass spectrometric analysis after proteolytic digestions. Two differently derivatized SV-IV molecular forms, both possessing only one glutamine residue out of two (Gln-86) cross-linked to endogenous lysine residues, were produced when spermidine was omitted from the reaction mixture: (i) an insoluble homopolymer in which Lys-2, -4, -59, -78, -79, and -80 were involved…

MaleTissue transglutaminaseSeminal Plasma ProteinsLysineGuinea PigsMolecular Sequence DataBiochemistryMass SpectrometrySubstrate SpecificityResidue (chemistry)chemistry.chemical_compoundAnimalsAmino Acid Sequencechemistry.chemical_classificationIsopeptide bondTransglutaminasesbiologyHydrolysisSeminal Plasma ProteinsProstatic Secretory ProteinsProteinsSeminal VesiclesRats Inbred StrainsRatsSpermidineSecretory proteinchemistryBiochemistryLiverbiology.proteinPolyamineChromatography LiquidBiochemistry
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